Frequently Asked Questions (FAQ)

• How does the Trinocular feature benefit advanced imaging in fluorescence?
  A: The dedicated Trinocular port ensures that a high-resolution camera can be permanently mounted and optimally aligned. In fluorescence imaging, where light intensity is often low, this stable alignment is crucial for maximizing light capture and securing sharp, high-quality digital micrographs without interrupting the user's viewing path.
• What is the advantage of using Plan Achromatic Fluorescence Objectives?
  A: These objectives are crucial because they are specifically manufactured to minimize their own background fluorescence (**auto-fluorescence**) and ensure a flat field of view (**Plan**). This results in maximum signal-to-noise ratio, providing much brighter and clearer fluorescent signals against a truly dark background.
• Can the XD system be upgraded from a Mercury lamp to an LED fluorescence source?
  A: Yes, many modern XD fluorescence microscopes are modular. While older models used Mercury lamps, many contemporary systems offer high-power LED light sources. LEDs are preferred due to their long life, stability, immediate on/off capability, and lack of toxic materials, often allowing for easy conversion.
• Is the focusing system stable enough for live cell imaging?
  A: Yes, the XD series features a robust Co-axial Coarse and Fine Focusing mechanism with tension adjustment. This stability prevents "focus drift," which is essential for capturing long-duration time-lapse sequences in live cell fluorescence studies.
• Which types of specimens are best observed under this microscope?
  A: It is ideal for samples stained with fluorescent dyes, such as cells or tissues labeled for specific proteins (Immunofluorescence), DNA/RNA analysis (FISH), bacterial identification, and observing naturally fluorescent materials like chlorophyll or certain minerals.