In-Depth Scientific Analysis and Utility

Customer Inquiries (FAQ)

• 1. Why is an Inverted design necessary for cell culture?
  The **Inverted design** places the objectives beneath the stage. This allows you to view samples through the bottom of thick culture vessels (like Petri dishes or multi-well plates) that cannot be inverted, ensuring the immersion media remains on top and the objectives stay dry and clear.
• 2. How many filter sets are included, and which ones are standard?
  The microscope typically comes with a **multi-position fluorescence filter turret**. Standard high-demand filter sets often include **FITC** (for green emission), **TRITC** (for red emission), and **DAPI** (for blue/UV excitation, used for nuclear staining). Additional filter blocks can be easily added based on specific research needs.
• 3. What does "LWD Objective" mean, and why is it important here?
  **LWD** stands for **Long Working Distance**. These objectives are essential for inverted microscopy because they allow enough space (working distance) between the objective lens and the coverslip to accommodate the thick base of the culture flask or dish.
• 4. Can this microscope perform Brightfield and Phase Contrast?
  Yes. While its primary function is fluorescence, it includes **Transmitted Illumination** capability with a dedicated condenser, allowing for standard Brightfield and often a **Phase Contrast** option (with appropriate Phase objectives and rings) for general cell inspection before fluorescence imaging.
• 5. What kind of light source is recommended for reliable fluorescence?
  For routine research, high-intensity **Mercury Arc Lamps** are common. However, modern systems increasingly use long-life, stable **High-Power LED Light Sources**, offering specific wavelength control, immediate on/off capability, and superior safety without the hazardous waste of mercury.